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Journal Entry:

Did the plasmid in the vial glow green before being inserted into the bacteria? Why?

Learning Intentions

• We will learn how Bacteria Transformation takes place and the steps that must take place for it to be successful.

• We will learn how to explain the role of a plasmid in Bacteria Transformation and how it can be used to create antibiotic resistant bacteria

• We will learn sterile technique and understand its importance in certain data collection.

Closing Task:

You can relate results from our lab to use of the pGLO gene in scientific research outside of our classroom

You can apply what you learned in the lab to answer a released FRQ.

Content Standards being covered:

Topics:
IST-1.P Explain the use of genetic engineering techniques in analyzing and manipulating DNA.

Science Practices:
Explain the relationship between experimental results and larger biological concepts, processes, or theories.

Habits of Mind/Life skills being covered:

Thinking and Communicating with Clarity

Making Connections with Past Knowledge

Taking Responsible Risks